r gnavus atcc 35913 strain Search Results


r gnavus 35913  (ATCC)
96
ATCC r gnavus 35913
R Gnavus 35913, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+gnavus+atcc+35913+strain/Ruminococcus+gnavus+Moore+et+al/10__1016_slash_j__carbpol__2024__122754-112-15-10
Average 96 stars, based on 1 article reviews
r gnavus 35913 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
ruminococcus gnavus atcc 29149  (ATCC)
96
ATCC ruminococcus gnavus atcc 29149
Ruminococcus Gnavus Atcc 29149, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+gnavus+atcc+35913+strain/Ruminococcus+gnavus/pmc08631720-191-1-3
Average 96 stars, based on 1 article reviews
ruminococcus gnavus atcc 29149 - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
mek6  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology mek6
The ECS cell phenotype and MEK3/6 and p38 signaling. A-C) Cells were electroporated with 3 μg of Control-, MEK3-, or <t>MEK6-siRNA</t> and the impact on p38 activity, and spheroid formation and invasive, were measured. D) Cells were electroporated with 3 μg of Control-, MEK3-, or MEK6-siRNA, grown for 5 d as spheroids, and cell extracts (300 μg protein) were assayed for ability to enhance HUVEC tube formation as measured by junction, segment and node formation. E) Cells were electroporated with 3 μg of the indicated siRNA and p38 level and activity were measured at 48 h. F and G) Impact of p38 knockdown on spheroid formation and matrigel invasion. H and I) Treatment with SB203580, a p38α/β inhibitor, suppresses ECS cell spheroid formation and invasive potential. J and K) RhoA restores the ECS cell phenotype in NRP-1 knockdown cells. SCC-13 derived NRP-1 knockout cells were electroporated with 3 μg of empty vector or RhoA WT expression plasmid, and plated for spheroid formation (5 d). The immunoblot monitors NRP-1 knockdown, RhoA overexpression, and the impact on p38T and p38-P level. SCC-13 and SCC13-NRP-1-KOc8 cells were electroporated with 3 μg of the indicated plasmids and ability to form spheroids was monitored at 5 d. The asterisk indicates a significant reduction in spheroid formation in SCC13-NRP1-KOc8 cells versus SCC-13. The double asterisk indicates an increase in spheroid formation in RhoA WT expression plasmid-electroporated versus empty vector-electroporated SCC13-NRP1-KOc8 cells, n = 3, P ≤ 0.005. All bars = 100 μm. [Color figure can be viewed at wileyonlinelibrary.com]
Mek6, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+gnavus+atcc+35913+strain/MEK-6+siRNA/pmc06417965-111-51-53
Average 90 stars, based on 1 article reviews
mek6 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
pcdna wnt6 vector  (Addgene inc)
93
Addgene inc pcdna wnt6 vector
The ECS cell phenotype and MEK3/6 and p38 signaling. A-C) Cells were electroporated with 3 μg of Control-, MEK3-, or <t>MEK6-siRNA</t> and the impact on p38 activity, and spheroid formation and invasive, were measured. D) Cells were electroporated with 3 μg of Control-, MEK3-, or MEK6-siRNA, grown for 5 d as spheroids, and cell extracts (300 μg protein) were assayed for ability to enhance HUVEC tube formation as measured by junction, segment and node formation. E) Cells were electroporated with 3 μg of the indicated siRNA and p38 level and activity were measured at 48 h. F and G) Impact of p38 knockdown on spheroid formation and matrigel invasion. H and I) Treatment with SB203580, a p38α/β inhibitor, suppresses ECS cell spheroid formation and invasive potential. J and K) RhoA restores the ECS cell phenotype in NRP-1 knockdown cells. SCC-13 derived NRP-1 knockout cells were electroporated with 3 μg of empty vector or RhoA WT expression plasmid, and plated for spheroid formation (5 d). The immunoblot monitors NRP-1 knockdown, RhoA overexpression, and the impact on p38T and p38-P level. SCC-13 and SCC13-NRP-1-KOc8 cells were electroporated with 3 μg of the indicated plasmids and ability to form spheroids was monitored at 5 d. The asterisk indicates a significant reduction in spheroid formation in SCC13-NRP1-KOc8 cells versus SCC-13. The double asterisk indicates an increase in spheroid formation in RhoA WT expression plasmid-electroporated versus empty vector-electroporated SCC13-NRP1-KOc8 cells, n = 3, P ≤ 0.005. All bars = 100 μm. [Color figure can be viewed at wileyonlinelibrary.com]
Pcdna Wnt6 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/r+gnavus+atcc+35913+strain/pcDNA-Wnt6+(Plasmid+%2335913)/pmc06160775-143-5-12
Average 93 stars, based on 1 article reviews
pcdna wnt6 vector - by Bioz Stars, 2026-10
93/100 stars
  Buy from Supplier

Image Search Results


The ECS cell phenotype and MEK3/6 and p38 signaling. A-C) Cells were electroporated with 3 μg of Control-, MEK3-, or MEK6-siRNA and the impact on p38 activity, and spheroid formation and invasive, were measured. D) Cells were electroporated with 3 μg of Control-, MEK3-, or MEK6-siRNA, grown for 5 d as spheroids, and cell extracts (300 μg protein) were assayed for ability to enhance HUVEC tube formation as measured by junction, segment and node formation. E) Cells were electroporated with 3 μg of the indicated siRNA and p38 level and activity were measured at 48 h. F and G) Impact of p38 knockdown on spheroid formation and matrigel invasion. H and I) Treatment with SB203580, a p38α/β inhibitor, suppresses ECS cell spheroid formation and invasive potential. J and K) RhoA restores the ECS cell phenotype in NRP-1 knockdown cells. SCC-13 derived NRP-1 knockout cells were electroporated with 3 μg of empty vector or RhoA WT expression plasmid, and plated for spheroid formation (5 d). The immunoblot monitors NRP-1 knockdown, RhoA overexpression, and the impact on p38T and p38-P level. SCC-13 and SCC13-NRP-1-KOc8 cells were electroporated with 3 μg of the indicated plasmids and ability to form spheroids was monitored at 5 d. The asterisk indicates a significant reduction in spheroid formation in SCC13-NRP1-KOc8 cells versus SCC-13. The double asterisk indicates an increase in spheroid formation in RhoA WT expression plasmid-electroporated versus empty vector-electroporated SCC13-NRP1-KOc8 cells, n = 3, P ≤ 0.005. All bars = 100 μm. [Color figure can be viewed at wileyonlinelibrary.com]

Journal: Molecular carcinogenesis

Article Title: NRP-1 interacts with GIPC1 and SYX to activate p38 MAPK signaling and cancer stem cell survival

doi: 10.1002/mc.22943

Figure Lengend Snippet: The ECS cell phenotype and MEK3/6 and p38 signaling. A-C) Cells were electroporated with 3 μg of Control-, MEK3-, or MEK6-siRNA and the impact on p38 activity, and spheroid formation and invasive, were measured. D) Cells were electroporated with 3 μg of Control-, MEK3-, or MEK6-siRNA, grown for 5 d as spheroids, and cell extracts (300 μg protein) were assayed for ability to enhance HUVEC tube formation as measured by junction, segment and node formation. E) Cells were electroporated with 3 μg of the indicated siRNA and p38 level and activity were measured at 48 h. F and G) Impact of p38 knockdown on spheroid formation and matrigel invasion. H and I) Treatment with SB203580, a p38α/β inhibitor, suppresses ECS cell spheroid formation and invasive potential. J and K) RhoA restores the ECS cell phenotype in NRP-1 knockdown cells. SCC-13 derived NRP-1 knockout cells were electroporated with 3 μg of empty vector or RhoA WT expression plasmid, and plated for spheroid formation (5 d). The immunoblot monitors NRP-1 knockdown, RhoA overexpression, and the impact on p38T and p38-P level. SCC-13 and SCC13-NRP-1-KOc8 cells were electroporated with 3 μg of the indicated plasmids and ability to form spheroids was monitored at 5 d. The asterisk indicates a significant reduction in spheroid formation in SCC13-NRP1-KOc8 cells versus SCC-13. The double asterisk indicates an increase in spheroid formation in RhoA WT expression plasmid-electroporated versus empty vector-electroporated SCC13-NRP1-KOc8 cells, n = 3, P ≤ 0.005. All bars = 100 μm. [Color figure can be viewed at wileyonlinelibrary.com]

Article Snippet: For electroporation, 1 × 10 6 cells were electroporated with 3 μg of control- (D-001206–13-05, Dharmacon), VEGF-A (sc-29520, Santa Cruz), NRP-1 (sc-36038, Santa Cruz), GIPC1 (M-019997–02-005, Dharmacon), p38α (sc-29433, Santa Cruz), p38β (sc-39116, Santa Cruz), p38γ (sc-39118, Santa Cruz), p38δ (sc-36456, Santa Cruz), MEKK1 (sc-35898, Santa Cruz), MEK3 (sc-35907, Santa Cruz), MEK6 (sc-35913, Santa Cruz), Syx (Dharmacon, M-013873–01-0005) or RhoA (Santa Cruz, sc-29471) siRNA using the Amaxa electroporator and the VPD-1002 nucleofection kit (Germany).

Techniques: Control, Activity Assay, Knockdown, Derivative Assay, Knock-Out, Plasmid Preparation, Expressing, Western Blot, Over Expression